BioCision LLC today released new data demonstrating that the company’s CoolCell® technology resulted in a 33 percent increase in human embryonic stem cell (hESC) viability when compared to common freezing methods, such as styrofoam boxes, paper towel wraps and alcohol-based methods.
BioCision's CoolCell. (Photo: Business Wire)
Based on thermo-conductive principles and developed through precision-engineering, CoolCell is a rate-controlled cryopreservation system that ensures uniform and reproducible freezing rates for biological samples, including primary cells, cell lines, stem cells, yeast and other cell types.
“Addressing the lack of standardization in common sample handling, processing and viable long-term storage has emerged as one of the greatest challenges facing biobanks, diagnostics companies and researchers today,” said Rolf Ehrhardt, CEO, BioCision. “Scientists know that error is inherent in sample handling and can be the difference between viable and non-viable cells, particularly when handling embryonic stem cells that are extremely fragile following thawing.”1
Researchers set out to compare cell recovery rates post-thaw using the CoolCell method of cryopreservation and three commonly used methods with two human Embryonic Stem Cell (hESC) lines, RC-7 and RC-10. The study concluded that:
The study was conducted in collaboration with scientists at Edinburgh-based Roslin Cellab, a stem cell technology company with expertise in the stem cell culture and regenerative medicine field, and sister company to Roslin Cells, a leading European and UK provider of clinical grade human embryonic stem cells.
“The results of our study show that the CoolCell technology clearly outperforms all other methods and provides excellent efficiency and reproducibility for stem cell cryopreservation and recovery,” said Aidan Courtney, Director at Roslin Cellab.
CoolCell alcohol-free cell cryopreservation systems provide highly reproducible controlled-rate freezing for eukaryotic cells, including primary cells, cell lines, stem cells, PBMC, yeast and other types of cells. Used worldwide in academic, research and clinical laboratories, CoolCell is the standard for passive controlled-rate cell cryopreservation. The CoolCell technology - using novel thermo-conductive alloy and highly insulative materials - ensures precise heat removal from each tube and standardizes the freezing experience of all cryogenic tubes in a -80°C freezer.
BioCision’s mission is to standardize pre-analytical sample handling. They develop novel products that eliminate variability in common, often-overlooked, laboratory procedures. Their products are marketed globally and are widely used by the leading institutions in the biotech, pharmaceutical, clinical, diagnostic, academic and government scientific community. BioCision’s products include CoolRack®, CoolSink™ and ThermalTray™ laboratory tube and plate temperature standardizing modules, CoolBox™ ice-free benchtop coolers, CoolCell® alcohol-free cell freezing containers and TruCool™ accessories and consumables.
1 hESC Culture Protocols. Manual for Essential Human Embryonic Stem Cell Culture Methods Course. Accessed on 3/22/2012 at http://www.stemcell.umn.edu/prod/groups/med/@pub/@med/@stemcell/documents/content/med_content_129984.pdf.
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Last updated on: 27/03/2012