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10-Sep-2021

SH-SY5Y Cell Line Culture Protocol and Research Applications

SH-SY5Y Cell Line Culture Protocol and Research Applications

Summary

The SH-SY5Y cell line is in the widespread use in experimental neurological studies, including the analysis of neuronal differentiation, metabolism, functions related to neurodegenerative and neuroadaptive processes, neurotoxicity, and neuroprotection.
  • Author Name: AcceGen R&D Team
Editor: BANBAN LIU Last Updated: 10-Sep-2021

Background

SH-SY5Y cells, the subline of the parental line SK-N-SH cells, are originally established from a bone marrow biopsy of a neuroblastoma patient with sympathetic adrenergic ganglial origin. SK-N-SH were subcloned three times: first to SH-SY, and then to SH-SY5, finally to SH-SY5Y. SH-SY5Y were deposited to the ATCC® in 1970 by June L. Biedler. Once the primary mammalian neurons derived from embryonic central nervous system tissue terminally differentiate into mature neurons, the cells can be no longer propagated. While the application of SH-SY5Y overcomes this limitation [1].

 

The SH-SY5Y cell line is a comparatively homogeneous neuroblast-like cell line, which exhibits neuronal marker enzyme activity (tyrosine and dopamine-β-hydroxylases), specific uptake of norepinephrine (NA), and expresses one or more neurofilament proteins. SH SY5Y cells also express opioid, muscarinic, and nerve growth factor receptors. In addition, SH-SY5Y cells are able to proliferate in culture for long periods without contamination, which is a prerequisite for in vitro cell model developing. Compared with the large-scale expansion before differentiation, the culture is relative easy and low cost to culture compared to primary neurons. The ethical concerns associated with primary human neuronal culture are not involved as these cells are considered a cell-line. Besides, as they are human-derived, SH-SY5Y neuroblastoma cells express a number of human-specific proteins and protein isoforms which are not inherently present in rodent primary cultures. Moreover, differentiation synchronizes the cell cycle. The cell cycle of undifferentiated shsy5y cells and other commonly used cell lines can drastically fluctuate, so as to produce a homogenous neuronal cell population. Thus, SH-SY5Y cells widely used in neurobiology research [2].

 

 

Figure 1. Undifferentiated SH-SY5Y cells. Cells tend to grow in clusters and may form clumps of rounded cells on top of one another (arrow). At edges of the cluster, cells begin to extend short neurites (arrowhead)

 

 

Figure 2. Differentiated SH-SY5Y cells. Cells do not cluster and have a more pyramidal shaped cell body (arrowhead). Neurites begin to extend, reminiscent of dendrites and/or axons

 

SH-SY5Y Culture Protocol

1. Reagents

Dulbecco’s Minimum Essential Medium (DMEM) (DMEM is most commonly used, followed by DMEM/F12, MEM/F12).

Fetal Bovine Serum (FBS), 10% final concentration.

Penicillin/Streptomycin (Pen/Strep), 1 % final concentration (100 IU/ml, 100 μg/ml, respectively).

GlutaMAX, 2% final concentration.

Trypsin/EDTA.

 

2.Methods

Prepare growth medium: DMEM medium (10% FBS, 2% GlutaMAX and 1% pen/strep)

Cell recovery: Obtain cells and thaw them quickly at 37 °C. Gently remove cell suspension from tube and add to a petri dish containing warm (37 °C) growth medium. Culture cells at 37 °C, 5 % CO2. Growth medium should be refreshed every 3–5 days. Monitor cells for confluence. When cells reach 80–90 % confluence, subculture as described below.

Cell subculture: Aspirate medium under sterile conditions. Rinse adherent cells once with sterile 1× PBS pre-warmed to 37 °C or room temperature. To prevent detaching cells, add the PBS to the inner wall of the petri dish that doesn’t have cells attached. Do not add PBS directly onto cell monolayer. Gently tip the flask so that the PBS washes over the cell monolayer. Aspirate PBS. Add trypsin to adherent cells for approximately 2 min or until cells visibly detach from petri dish. Neutralize trypsin by adding an equal volume of DMEM medium containing 10 % FBS. Collect detached cell suspension and centrifuge at 1,000 rpm for 5 min at room temperature to concentrate cell pellet. Aspirate supernatant carefully without disturbing the cell pellet. Gently suspend pellet in DMEM medium containing 10 % FBS. To separate cells from clumps, pipette up and down gently until the suspension appears homogenous. Count cells using a hemocytometer and plate at approximately 3 × 103 to 1 × 105 cells/cm2. Culture cells at 37 °C, 5 % CO2. Growth medium should be refreshed every 3–5 days. Monitor cells for confluence. When cells reach 80–90 % confluence, subculture as described below.

Freezing Down: Harvest 80–90 % confluent monolayer and pellet cells as described above. Gently suspend the cell pellet in 1 ml of 90 % FBS, 10 % DMSO in a sterile 1.5 ml screw cap vial appropriate for storage in the vapor phase of liquid nitrogen. Store cells at −80 °C for approximately 24 h in an insulated cryobox and then transfer tubes to liquid nitrogen for long-term storage.

Notes: 1) Recommendations on the composition of the growth medium for SH-SY5Y cell proliferation vary by cell line distributors.

2) Air will reduce the survival rate of cells. Therefore, be careful not to introduce air to flasks or tubes when pipetting/transferring cells.

3) The color of the medium indicates the metabolic rate of the key components of cells. When medium becomes more acidic (color looks more yellow than red), it may be the time to change the medium.

4) Rinsing with PBS to remove the majority of the serum contained in growth medium. Trypsin works more efficiently in the absence of serum as serum would inhibit its activity.

5) Reduce the time cells are exposed to trypsin. After 1 min exposure, you may tap the flask gently to assist in detachment.

6) You will find that SH-SY5Y cells need to be plated at a density conducive to cell–cell communication in order to proliferate. If cells are plated too sparsely, the growth rate will be reduced and the cell death rate will be high [1,2].

 

Application of SH-SY5Y

SH-SY5Y cell line is widely used in laboratory medical scientific research, such as drug development and Parkinson’s Disease research, based on the advantages of its human origin, catecholaminergic (though not strictly dopaminergic) neuronal properties, and easy maintenance [2].

Parkinson’s Disease Research

Parkinson’s Disease (PD) is an age-related progressive neurodegenerative disorder with a prevalence of 1%-2% in people over the age of 50. Pathologically, PD is characterized by the loss of mesencephalic dopaminergic (mDA) neurons. Because of its prevalence and lack of effective treatment, PD is a major societal health problem. Therefore, the development of a stable and reliable dopaminergic neuronal cell model is particularly necessary for studying the pathogenesis of PD and developing therapeutic strategies [2].

 

SH-SY5Y has become a popular cell model for PD research as it possesses many characteristics of dopaminergic neurons (DAergic neurons). For instance, SH-SY5Y cells express tyrosine hydroxylase, dopamine-beta-hydroxylase, and the dopamine transporter. Furthermore, SH-SY5Y neuronal cells can differentiate into a functionally mature neuronal phenotype under the catalysis of various reagents. SH-SY5Y cells would stop proliferating and maintain a constant cell number after differentiation. Additionally, different neuronal phenotypes and biochemical changes are produced under the induction of different differentiating agents. For example, SH-SY5Y cells can differentiate into a cholinergic neuronal phenotype under the induction of retinoic acid, which can increase the susceptibility of SH-SY5Y cells to neurotoxins and neuroprotective agents. Whereas treatment with retinoic acid followed by phorbol ester 12-O-tetradecanoylphorbol-13-acetate can induce a DAergic neuronal phenotype and decrease the susceptibility of cells to neurotoxins and neuroprotective agents. Some differentiating agents also alter kinetics of 1-methyl-4-phenyl-pyridinium (MPP(+)) uptake, making SH-SY5Y cells more similar to primary mesencephalic neurons. Thus, researchers are supposed to select the most appropriate cell differentiation type based on the research purpose [3].

 

Drug Testing

SH-SY5Y cell line has also been applied to research and development trials in the field of neuro-related drugs.

 

1-methyl-4-phenylpyridinium (MPP+), 6-hydroxydopamine (6-OHDA) and rotenone are the most frequently used compounds in drug-based approaches, as they can dysregulate multiple cellular pathways, focusing on mitochondrial dysfunction and oxidative stress [3].

 

In a study published in the journal Environment International in 2020, human neuroblastoma SH-SY5Y cells are used to evaluate effects on oxidative stress, neuronal development and cell death signaling pathways under the induction of glyphosate and its metabolite aminomethylphosphonic acid (AMPA). Their results indicated that glyphosate and AMPA had cytotoxic effects on neuronal development, oxidative stress and cell death through apoptotic, autophagy and necrotic pathways, which confirmed that glyphosate environmental exposure becomes a concern. The study demonstrates that SH-SY5Y cell line could be considered as an in vitro system for pesticide screening [4].

 

SH-SY5Y cell lines have also been widely used in several other drug studies, for example, the research on the antitumor activity of erythromycin on human neuroblastoma cell line. Exposing SH-SY5Y cells to erythromycin at different concentrations for different durations obtained the different proliferation results, from which can be drawn that erythromycin could restrain the proliferation of SH-SY5Y cells. The antitumor mechanism of erythromycin may involve the regulation of the expression of c-Myc and p21 (WAF1/Cip1) proteins [5].

 

Some researchers used SH-SY5Y cell line to study the anti-tumor effect of angelica sinensis polysaccharide (AP). They aimed to explore the effects of AP on SH-SY5Y neuronal cells and its underlying mechanisms. In summary, AP was first identified as inhibiting proliferation, migration, and invasion but inducing apoptosis. AP might inhibit the tumorigenesis of SH-SY5Y cells through miR-675-mediated inactivation of the PI3K/AKT and JAK/STAT pathways. And KIF1Bβ may be a target of miR-675[6].

 

Future Perspective

In conclusion, the SH-SY5Y cell line is in the widespread use in experimental neurological studies, including the analysis of neuronal differentiation, metabolism, functions related to neurodegenerative and neuroadaptive processes, neurotoxicity, and neuroprotection.

 

Both differentiated and undifferentiated SH-SY5Y cells are commonly used as a DAergic neuron cell model for PD research. The use of some differentiating agents makes SH-SY5Y cells more potential for neurotoxicity and neuroprotection studies, increasing the relevance to PD experimental research. In the past two decades, a variety of methods to differentiate SH-SY5Y cells have been evaluated, but the development of an optimal differentiated SH-SY5Y DAergic cell model for PD research still needs the further study [2].

 

Where to get SH-SY5Y and other Human Neuroblastoma Cell Lines?

AcceGen provides undifferentiated SH-SY5Y and marked cell line like Green fluorescent alpha-synuclein SH-SY5Y Cell line for any specific research needs. Other Human Nerve Tumor Cell Lines such as SK-N-SH are also available in AcceGen.

 

It is our pleasure to help relative researches to move forward. All the products of AcceGen are strictly comply with international standards. For more detailed information, please visit our product portfolio or contact inquiry@accegen.com.