Photometrics, Cairn Research and Mizar Imaging Announce New Collaboration for High Power, High Sensitivity Light Sheet Fluorescence Microscopy

US-based scientific camera designer and manufacturer Photometrics, scientific instruments manufacturer Cairn Research and systems developer Mizar Imaging have created a partnership to offer a new, high power, high sensitivity light sheet fluorescence microscopy solution. The MizarTILT light sheet system combined with the Photometrics Prime 95B, the world's first back-illuminated scientific CMOS camera, provides an ideal solution for researchers looking to use light sheet fluorescence microscopy for long time scale imaging of coverslip-mounted samples with high NA Plan Apo and TIRF objectives.

The MizarTILT is a light sheet conversion system for fluorescence microscopes that gives users light sheet illumination capabilities along with the high-resolution imaging advantages of epifluorescence. The TILT uses a cylindrical lens to create a light sheet from an expanded laser source, similar to a conventional light sheet system. The critical difference is the TILT directs the light sheet at an angle to focus the thinnest part of the light sheet as close as possible to the objective lens. This allows researchers to use high NA objectives and to benefit from higher resolution imaging and more efficient light-gathering.

Working together with the Photometrics Prime 95B™ Scientific CMOS camera, images can be obtained on the TILT with a 95% quantum efficiency to maximize the light-gathering effectiveness of high power objectives. Furthermore, high power objectives complement the large 11x11 μm pixels of the Prime 95B to allow for high magnification, high-resolution imaging. This is all possible with the fast speed and large field of view expected of a scientific CMOS device.

The combination of the MizarTILT and the Photometrics Prime 95B is perfect for any researcher who wants to use light sheet imaging with higher power objectives to greatly reduce photodamage and photobleaching and increase imaging time.