Articles

Nanopore full-length transcriptome sequencing is an innovative technique that utilizes Oxford Nanopore Technologies' (ONT) sequencing platform to obtain comprehensive and uninterrupted high-quality full-length sequences of transcripts. This cutting-edge approach enables researchers to accurately identify various structural variations within transcripts, including alternative splicing, gene fusion, selective polyadenylation of alternative polyadenylation sites (APAs), allele-specific expression, and other alterations in transcript structure. Additionally, this method facilitates precise quantification of transcript expression levels, encompassing both messenger RNA (mRNA) and polyA+ long non-coding RNA (lncRNA).
Nanopore full-length transcriptome sequencing is an innovative technique th…

Exosomes are small extracellular vesicles that contain RNA, proteins, and lipids. These vesicles play a crucial role in intercellular communication and have been implicated in various physiological and pathological processes. Exosome RNA-Seq is a powerful technique that can be used to study the RNA content of exosomes and gain insight into their function and regulation. In this guide, we will provide an overview of the key steps involved in exosome RNA-Seq and highlight some important considerations for beginners.
Exosomes are small extracellular vesicles that contain RNA, proteins, and lipids. These vesicles play a crucial role in intercellular communication and have been implicated in various physiological and pathological processes. Exosome RNA-Seq is a powerful technique that…

The quantification and analysis methods of exosomal RNA are also key factors in exploring the dynamic changes of exosomal RNA in the tumor microenvironment. Currently, commonly used methods for quantifying and analyzing exosomal RNA include qPCR, sequencing, microarray, etc.
The Relationship Between Exosomes and Cancer Exosomes are nanoscale vesicles derived from intracellular lysosomes that shuttle a variety of biomolecules, such as nucleic acids, proteins, lipids, amino acids and metabolites, and play a key role in regulating intercellular communication. Exosomes can be secreted by almost all types of cells, including immune cells, blood cells, neuronal cells, epithelial cells, and cancer cells. Exosomes contain various types of RNA molecules, including miRNA, mRNA, and even long non…

Epigenetics has been defined as the study of stable alterations in gene expression potentials that arise during development and cell proliferation, or alterations in DNA function without alterations in DNA sequence. Modern epigenetic features refer to the alteration of DNA and/or associated proteins without nucleotide sequence variance, which transmits the data contained to the next generation. It has been considered that diseases are generally influenced by obtained biological alterations, but it is becoming evident that any phenotype is the result of a complex interplay between genotype, epigenome, and environment. Epigenetics concentrates on pathways that control how and when specific genes are turned on and/or off, while epi-genomics assess epigenetic changes in a cell or whole organism across many genes.

Oxford Nanopore Technologies (ONT) sequencing has witnessed significant progress in recent years, becoming a key player in the genomics field. As the technology matures, so does the bioinformatics analysis of ONT data. Researchers have been diligently developing specialized tools and algorithms to better utilize the unique characteristics of ONT data, such as long read lengths and ionic current signals. This article explores the latest bioinformatics advancements that enable enhanced base calling, base modification detection, error correction, assembly, and alignment of ONT data.
Oxford Nanopore Technologies (ONT) sequencing has witnessed significant progress in recent years, becoming a key player in the genomics field. As the technology matures, so does the bioinformatics analysi…

Cell cycle checkpoints are essential for maintaining genomic stability and preventing cancer. By understanding how cell cycle checkpoints work, scientists can develop new ways to treat cancer and other diseases.
The cell cycle is a complex process that involves the growth and division of a cell. It is divided into four phases: G1, S, G2, and M. During each phase, the cell undergoes a series of events that prepare it for the next phase. Cell cycle checkpoints are control mechanisms that ensure that the cell cycle proceeds in a timely and orderly manner.   There are three major cell cycle checkpoints: G1 checkpoint: This checkpoint occurs at the end of the G1 phase. It ensures that the cell has grown to a sufficient size and that all of the necessary nutrients and components are pr…

In order to fully understand the catalytic activity of enzymes, researchers have conducted a wide range of MD simulation studies on several types of enzyme catalytic processes and discussed the whole process in detail.
Enzyme catalysis includes complex processes such as the transport of substrates to the active area, selective catalysis of chemical reactions, and product release. Any chemical or non-chemical process may be a crucial step in determining enzyme activity due to the complex environmental effects of proteins. Molecular dynamics (MD) method is a motion equations of the multi-particle system developed based on Newtonian classical mechanics. MD simulates the microscopic process of the system over time to obtain the particle phase trajectory of the system, and studies the equi…

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845 Clinical Research Jobs Today - Search Now - https://bit.ly/47PzAY8. For nearly 25 years, PharmiWeb has been bringing you the latest clinical research jobs from the best Employers and Agencies across Europe and the USA. Search the latest job vacancies here:   - Clinical Research Associate (261) - https://bit.ly/40XQQIo - Clinical Trials Manager / Administrator (114) - https://bit.ly/3T16AZ6 - Clinical Operations (96) - https://bit.ly/3sQNSJf - Clinical Project Manager (59) - https://bit.ly/47QWYEt - Study Start Up (58) - https://bit.ly/3T1W0RD - Clinical Development (37) - https://bit.ly/3R2pbSc - Pharmacovigilance (27) - https://bit.l…

Search 2,663 Jobs on PharmiWeb.jobs - the latest Life Science, Pharma, Biotech, Clinical Research jobs across Europe, the US and Australasia.
November 28th 2023., PharmiWeb.jobs, the leading job board for Life Sciences and pharma jobs, currently has 2,663 Pharma & Life Science job vacancies. Stay ahead in your Life Science career with up-to-date job listings, industry articles, and career advice. Search Now - https://bit.ly/46AN8Wg Clinical Research (851) - https://bit.ly/47NjLBP Sales / Commercial (224) - https://bit.ly/3N4CGPQ Quality (216) - https://bit.ly/3N5IHMu Data Management / Statistics (207) - https://bit.ly/3RfmOwx Regulatory Affairs (192) - https://bit.ly/3MZSIuz R & D (181) - https://bit.ly/3QX19bi Project Management (159) -…

The gene therapy market has emerged as a groundbreaking field in modern medicine, with a market size reaching USD 6.36 billion in 2023. This dynamic market is expected to experience rapid growth, projected at a Compound Annual Growth Rate (CAGR) of 22.8% during the forecast period from 2024 to 2032, ultimately reaching a staggering value of USD 40.39 billion by 2032.
The gene therapy market has emerged as a groundbreaking field in modern medicine, with a market size reaching USD 6.36 billion in 2023. This dynamic market is expected to experience rapid growth, projected at a Compound Annual Growth Rate (CAGR) of 22.8% during the forecast period from 2024 to 2032, ultimately reaching a staggering value of USD 40.39 billion by 2032. In this comprehensive guide, we will provide an in-depth expl…

The Patient Engagement Solutions Market is centered around the tools, technologies, and strategies employed to actively involve patients in their healthcare journey.
In the rapidly evolving landscape of healthcare, patient engagement has emerged as a pivotal focus area for healthcare providers and technology companies alike. The Patient Engagement Solutions Market has experienced remarkable growth and is poised for continued expansion. This in-depth guide aims to provide you with a comprehensive understanding of the Patient Engagement Solutions Market, including its overview, dynamics, trends, segmentation, growth drivers, recent developments, scope, analysis, COVID-19 impact, key players, and frequently asked questions (FAQs). Patient Engagement Solutions Market Overview: The Patient Enga…

Capillary Electrophoresis (CE) is an analytical technique used in the development and quality control of protein drugs. From medium optimization, clone screening, formulation stability research and purification process monitoring, to protein characterization, related impurity detection, protein structure identification and protein drug product quality control, CE is required in all aspects of protein drug development. For example, for protein purity determination, sodium dodecyl sulfate-capillary gel electrophoresis (CE-SDS) is better compared to SDS-PAGE. To determine protein isoelectric point, capillary isoelectric focusing (CIEF) is more accurate than the traditional gel strip method. For the characterization of glycosylation heterogeneity of glycoprotein drugs, CE can achieve high-resolution analysis.

In the realm of biological sciences, bile acids stand as pivotal molecules, playing a multifaceted role in various physiological processes within organisms. Bile acids are specialized molecules synthesized from cholesterol within the liver. These amphipathic compounds possess a hydrophilic region (carboxylic acid moiety) and a hydrophobic region (steroid backbone). Their distinctive structure enables them to serve as integral components in the process of lipid digestion and absorption.
In the realm of biological sciences, bile acids stand as pivotal molecules, playing a multifaceted role in various physiological processes within organisms. Bile acids are specialized molecules synthesized from cholesterol within the liver. These amphipathic compounds possess a hydrophilic region (carboxylic…

Metabolism is the assembly of biochemical reactions in living organisms, the material basis for life maintenance of living organisms, and an important basis for the study of life activities. Metabolomics studies the endogenous metabolic activities of life under the influence of the internal and external environments, including the detection and analysis of metabolite types and quantities and their change patterns, thus studying the nature of the occurrence and development of collective life activities.
Metabolism is the assembly of biochemical reactions in living organisms, the material basis for life maintenance of living organisms, and an important basis for the study of life activities. Metabolomics studies the endogenous metabolic activities of life under the influence of the internal a…

Plant hormones are trace organic compounds synthesized in plants that have significant effects on plant growth and development. They can be transferred from the site of production to the site of action. They can regulate plant growth and development at low concentrations, and are involved in almost every process of plant growth and development, from influencing cell division, elongation and differentiation to germination, rooting, flowering, fruiting, dormancy and abscission. Plant hormones mainly include ethylene, auxin, abscisic acid, gibberellins, cytokinins, jasmonates, brassinosteroids and salicylates, etc. In addition, some secondary metabolites, such as nitric oxide (NO) and recently discovered strigolactones, have also been suggested to act in a hormone-like manner in regulating plant growth and development.

Flow cytometry (FC) is an important tool for analyzing complex pathways and responses of single cells, which can track cell phenotypes and functions in multiple dimensions. It is also increasingly applied in drug development. FC not only can detect intracellular and extracellular components, but also detect soluble analytes in serum or plasma samples, such as cytokines, drug complexes, or anti-drug antibodies (ADA).
Flow cytometry (FC) is an important tool for analyzing complex pathways and responses of single cells, which can track cell phenotypes and functions in multiple dimensions. It is also increasingly applied in drug development. FC not only can detect intracellular and extracellular components, but also detect soluble analytes in serum or plasma samples, such as cytokines, dru…

Choosing the right eClinical vendor is the cornerstone of successful trials. An inadequate choice can lead to a cascade of challenges, from data inaccuracies to operational inefficiencies.
Choosing the right eClinical platform vendor is the cornerstone of successful trials. An inadequate choice can lead to a cascade of challenges, from data inaccuracies to operational inefficiencies. In this complex landscape, Clinion isn’t just a platform — it’s a seasoned guide adeptly navigating clinical trials with transparency, precision, and an innate understanding of the challenges. Now, let’s break down the core reasons why Clinion is the linchpin for your clinical trial success. Reason 1: User-Centric All the Way Clinion’s platform is designed with you in mind. From trial inception to completion…

Enzymes are proteins that catalyze or speed up chemical reactions in biological organisms, transforming target molecules into essential building blocks for life. In an industrial context, they help condense time-consuming processes, substitute environmentally harmful chemicals, and lower processing temperatures, saving both time and energy.
The role that industrial enzymes play in facilitating various processes in industries ranging from food and beverages to detergents, textiles, and biofuels is crucial yet often understated. These bio-catalysts are responsible for accelerating chemical reactions, making industrial processes more efficient, cost-effective, and eco-friendly. Despite their critical importance, the complexity around their production process is quietly intriguing and needs to…

Lysozyme, often touted as the body's natural antibiotic, is a powerful enzyme inherently produced in the mucosal linings of our body, including the saliva, tears, and milk. This enzyme plays a pivotal role in our immunity by breaking down the cell walls of harmful bacteria, thus acting as our body's frontline defense. With such a potent bacteriolytic capacity, the idea of harnessing its potential for food preservation is quite intriguing.
The field of enzymology is an ever-evolving realm teeming with perfectly crafted proteins called enzymes, each offering a unique functionality. One of the esteemed members of this field is the Lysozyme enzyme, specifically its food-grade variant. This enzyme boasts an impressive array of applications, making it a valuable asset in strengthening the quality…

Lysozyme, often touted as the body's natural antibiotic, is a powerful enzyme inherently produced in the mucosal linings of our body, including the saliva, tears, and milk. This enzyme plays a pivotal role in our immunity by breaking down the cell walls of harmful bacteria, thus acting as our body's frontline defense. With such a potent bacteriolytic capacity, the idea of harnessing its potential for food preservation is quite intriguing.
The field of enzymology is an ever-evolving realm teeming with perfectly crafted proteins called enzymes, each offering a unique functionality. One of the esteemed members of this field is the Lysozyme enzyme, specifically its food-grade variant. This enzyme boasts an impressive array of applications, making it a valuable asset in strengthening the quality…