Biotech - Articles

Next-generation sequencing, PacBio SMRT sequencing, and Nanopore sequencing, can generate numerous sequence data in a single run. Raw reads or assembled sequence need to be submitted to public sequence repository (DDBJ/ENA/GenBank - INSDC), which is required by the overwhelming majority of journals as accession numbers of theses sequence data should be presented in in the published papers. The submission portal (https://submit.ncbi.nlm.nih.gov/) is a programmatic interface for users to submit sequence data and download others’ sequence data.
Submission of sequence data to NCBI archives Next-generation sequencing, PacBio SMRT sequencing, and Nanopore sequencing, can generate numerous sequence data in a single run. Raw reads or assembled sequence need to be submitted to public seque…

Enter metagenomic next-generation sequencing (mNGS), an all-encompassing and precise approach for microbial detection and taxonomic characterization. While numerous studies and case reports underscore the efficacy of mNGS in enhancing the diagnosis, treatment, and tracking of infectious diseases, formidable obstacles still lie on the path forward.
  In recent years, the landscape of sequencing technologies has undergone a transformative shift, transitioning from research settings to the realm of clinical laboratories. This transformation has been catalyzed by rapid technological advancements and significant cost reductions. Despite a multitude of microorganisms known to inflict human infections, prevailing diagnostic techniques merely scratch the surface, leaving a vast array of pathog…

Protein labeling refers to the process of attaching a detectable tag or marker to a protein. This can be achieved through various chemical, enzymatic, genetic, or isotopic methods. The purpose of protein labeling is to make proteins traceable, enabling detailed analysis of their function, structure, and interactions within biological systems.
Protein labeling refers to the process of attaching a detectable tag or marker to a protein. This can be achieved through various chemical, enzymatic, genetic, or isotopic methods. The purpose of protein labeling is to make proteins traceable, enabling detailed analysis of their function, structure, and interactions within biological systems.   Labeling proteins plays a vital role in many biological experiments, particularly in understanding cellu…

Cell lysis refers to the process of breaking down the cell membrane to release cellular contents, including proteins, nucleic acids, and other biomolecules. Protein extraction is the subsequent step where proteins are separated from other cellular components for analysis or further manipulation. These processes are crucial for understanding cellular mechanisms, identifying potential biomarkers, and developing therapeutic strategies.
  Cell lysis refers to the process of breaking down the cell membrane to release cellular contents, including proteins, nucleic acids, and other biomolecules. Protein extraction is the subsequent step where proteins are separated from other cellular components for analysis or further manipulation. These processes are crucial for understanding cellular mecha…

Stable isotope labeling is a sophisticated technique that involves incorporating stable isotopes into molecules to trace and analyze various biological and chemical processes. Unlike radioactive isotopes, which decay over time and emit radiation, stable isotopes remain constant and do not pose the same safety risks. This stability allows for long-term studies and precise measurements without the complications associated with radiation.
What is Stable Isotope Labeling? Stable isotope labeling is a sophisticated technique that involves incorporating stable isotopes into molecules to trace and analyze various biological and chemical processes. Unlike radioactive isotopes, which decay over time and emit radiation, stable isotopes remain constant and do not pose the same safety risks. This stabi…

Therapeutic monoclonal antibodies (mAbs) are a class of antibody drugs that are highly uniform and have pharmacological effects against a single epitope obtained through molecular biology.
  Therapeutic monoclonal antibodies (mAbs) are a class of antibody drugs that are highly uniform and have pharmacological effects against a single epitope obtained through molecular biology.   In the process of new drug development, the study of pharmacokinetics is of great significance. The result can be used to guide the screening and development of drugs, and support the evaluation of their safety and the design of clinical drug delivery protocol.   Pharmacokinetic characteristics are composed of and determined by drug absorption, distribution, metabolism and excretion. The structure, ph…

Vaccines have been essential in disease prevention, and the requirements for vaccine quality continue to increase. The application of advanced separation and purification technologies during the preparation of traditional and new vaccines is an effective means to improve vaccine efficacy and reduce side effects.
Vaccines have been essential in disease prevention, and the requirements for vaccine quality continue to increase. The application of advanced separation and purification technologies during the preparation of traditional and new vaccines is an effective means to improve vaccine efficacy and reduce side effects. Here are some commonly used purification methods:   Centrifugal Ultracentrifugation The sample particles sink at a certain speed, determined by their size, shape, and…

Enzymes, biological catalysts integral to metabolic processes, have increasingly become central to numerous industrial applications. Their inherent specificity, efficiency under mild conditions, and eco-friendly nature present compelling advantages over conventional chemical catalysts, prompting widespread adoption in diverse industries. This article seeks to elucidate the multifaceted roles of enzymes in industrial processes, highlighting their biochemical mechanisms and potential for innovation and improvement.
Enzymes, biological catalysts integral to metabolic processes, have increasingly become central to numerous industrial applications. Their inherent specificity, efficiency under mild conditions, and eco-friendly nature present compelling advantages over conventional chemical cataly…

Ovalbumin, also known as albumin or OVA, is the most abundant protein found in egg white, accounting for approximately 54% of its total protein content. This versatile glycoprotein has captivated researchers and industry professionals alike due to its unique properties and wide-ranging applications. In this article, we'll explore the structure, functions, and diverse uses of ovalbumin in various fields.
  Ovalbumin, also known as albumin or OVA, is the most abundant protein found in egg white, accounting for approximately 54% of its total protein content. This versatile glycoprotein has captivated researchers and industry professionals alike due to its unique properties and wide-ranging applications. In this article, we'll explore the structure, functions, and diverse uses of ovalbumin…

Elastase is a powerful enzyme that plays a critical role in various biological processes, both in physiological and pathological contexts. It belongs to the family of serine proteases, which are enzymes characterized by a unique serine residue in their active site. This enzyme is known for its ability to degrade elastin, a key protein that provides elasticity and resilience to tissues such as skin, lungs, and blood vessels. Understanding the functions and implications of elastase is crucial in biochemistry and medicine, as it has a significant impact on both health and disease.
Elastase is a powerful enzyme that plays a critical role in various biological processes, both in physiological and pathological contexts. It belongs to the family of serine proteases, which are enzymes characterized…

Ribonucleases (RNases) represent a critical class of enzymes with a principal role in the degradation of RNA, crucial for maintaining cellular homeostasis and regulating gene expression. By cleaving RNA molecules, RNases are integral to numerous biological processes, ranging from RNA maturation to the innate immune response against RNA viruses. In this exploration, we delve into the multifaceted nature of ribonucleases, examining their structure, function, and potential in biotechnology.
Ribonucleases (RNases) represent a critical class of enzymes with a principal role in the degradation of RNA, crucial for maintaining cellular homeostasis and regulating gene expression. By cleaving RNA molecules, RNases are integral to numerous biological processes, ranging from RNA maturation to the innat…

Fluoropolymers, characterized by the replacement of hydrogen atoms with fluorine, offer unparalleled performance attributes that set them apart from other materials.
The Science of Fluoropolymers Fluoropolymers, characterized by the replacement of hydrogen atoms with fluorine, offer unparalleled performance attributes that set them apart from other materials. The C-F bond is the strongest single bond known in organic chemistry and has a strong polarity. The high chemical stability of fluoropolymers can also be explained by the bond strength of the central C-F bond, which can reach about 470 kJ/mol. In comparison, the strength of the C-C bond found in hydrocarbons is 350~380 kJ/mol. Types and Characteristics of Fluoropolymers The family of fluoropolymers encompasses several key var…

Producing microspheres involves sophisticated technologies to ensure uniform size, shape, and composition, making it essential to master both small-scale and mass production techniques.
Introduction to Microsphere Microspheres are small spherical particles ranging from 1 to 1000 micrometers in size. They are widely used in the pharmaceutical and biotechnology industries for drug delivery, controlled release systems, and other applications due to their unique properties and versatility. Producing microspheres involves sophisticated technologies to ensure uniform size, shape, and composition, making it essential to master both small-scale and mass production techniques. Small Batch Production of Microsphere Small batch production serves as the initial stage in microsphere manufacturing.…

Pharmaceutical CGMP manufacturing encompasses all processes that abide by these guidelines to ensure drug products meet required standards from raw material sourcing to final product distribution.
Introduction The pharmaceutical industry faces stringent regulatory requirements to ensure the safety, efficacy, and quality of drug products. Among these, Current Good Manufacturing Practice (CGMP) guidelines set by agencies such as the U.S. Food and Drug Administration (FDA) play a pivotal role. Pharmaceutical CGMP manufacturing encompasses all processes that abide by these guidelines to ensure drug products meet required standards from raw material sourcing to final product distribution. This article delves into the nuances of CGMP manufacturing, including pilot scale-up and commercial manufact…

Disease modeling involves creating representative systems that mimic the behavior and progression of infectious and noninfectious diseases in a controlled environment. AI’s comprehensive role in this process includes aiding in data processing, simulation and result interpretation. Even if only a handful of teams adopt it, their ability to process and generate results will accelerate progression throughout the field.
Artificial intelligence’s role in disease modeling could be transformative. Its ability to analyze massive datasets rapidly, uncover hidden trends and forecast likely outcomes could accelerate progression in the pharmaceutical industry. How can research teams use this technology to enhance, refine or reimagine their existing processes? How Does AI Fit Into Disease Modeling? Dise…

Roots analysis has released the latest market research report on the Pea Protein market, offering a comprehensive analysis of global industry size, and delving deeply into various segments covered in the study report.
Roots analysis has released the latest market research report on the Pea Protein market, offering a comprehensive analysis of global industry size, and delving deeply into various segments covered in the study report. Additionally, the report covers an in-depth study of current industry statistics, market size, regional insight, revenue share, growing segments, product portfolio, and production & distribution costs associated with the global Pea Protein Market. Besides growth insight, the market study report also identifies several factors influencing overall market growth…

This article offers an overview of nucleic acid vaccine adjuvant development, exploring its process, technological platforms, applications, and relevance.
Introduction Vaccines are pivotal in the prevention and control of infectious diseases. With advancements in biotechnology, nucleic acid vaccines, such as DNA and RNA vaccines, have emerged as a promising frontier in immunization strategies. Nucleic acid vaccines work by introducing genetic material into the body, instructing cells to produce antigens that trigger immune responses. To enhance the efficacy of these vaccines, adjuvants play an essential role. This article offers an overview of nucleic acid vaccine adjuvant development, exploring its process, technological platforms, applications, and relevance. What are Vaccine Adjuvants? V…

Veterinary APIs are the chemical substances within medications that produce the intended effects in treating animal diseases.
Introduction In the world of veterinary medicine, maintaining animal health is paramount, not only for the well-being of the animals but also to protect public health and ensure food safety. Integral to achieving this are veterinary active pharmaceutical ingredients (APIs) and their intermediates. These components are the backbone of effective veterinary drugs, providing the essential elements that combat diseases in animals. In this article, we dive deep into the significance of Veterinary APIs, the intricacies of their process development, and the customization of veterinary intermediates.   Importance of Veterinary APIs Veterinary APIs are the chemi…

In the realm of cosmetics, the integration of plant extracts has sparked significant interest due to their diverse benefits and natural origins.
In the realm of cosmetics, the integration of plant extracts has sparked significant interest due to their diverse benefits and natural origins.  Plant Extracts for Moisturizing Moisturizing is a fundamental aspect of skincare, and plant extracts play a pivotal role in this domain. The polysaccharides and glycosides in plant extracts contain hydroxyl groups in their structures, which enable them to retain water through hydrogen bonds. Most plant polysaccharides have hygroscopic properties, such as aloe polysaccharides, which are the main active ingredients of aloe extracts and are widely used moisturizing raw materials in cosmetics. In a…

The article gives an overview of four primary types of porous materials: activated carbon, zeolites, metal-organic frameworks (MOFs), and covalent organic frameworks (COFs).
Porous materials are materials that have small pores or cavities throughout their structure, allowing for the passage of fluids or gases. These materials, with their myriad of microscopic voids, have gained substantial attention in various fields such as catalysis, adsorption, and separation technologies due to their remarkable properties including large surface area, tuneable pore size, and distinctive porosity. In addition, they facilitate applications starting from energy storage to drug delivery systems. Porous materials could be organic or inorganic, with structures ranging from amorphous to crystalline, and sizes…