Biotech - Articles

Dual RNA sequencing is one of the novel RNA-seq technologies. It shines a light on the simultaneous transcriptomics in host-pathogen interactions and is particularly suitable for investigating the molecular mechanisms of hosts and pathogens interactions. Dual RNA-seq does not require predesigned species-specific probes and allows the detection of transcripts with low abundances in a sensitive manner.
What is dual RNA sequencing? Understanding how pathogen induces disease requires the knowledge on which genes are expressed and how they are regulated during infection. RNA sequencing has been routinely used to analyze the gene expression of microbial pathogens. Apart from the standard mRNA sequencing, new methods are surging based on RNA-seq, mapping the post-transcriptional networks control…

Bioinformatics is a discipline developed on the basis of biology, mathematics and computer science. It effectively acquires and analyzes biological data such as nucleic acid sequences and protein structures, so as to conduct comprehensive and accurate biological analysis. Bioinformatics is an extremely important tool to study the microorganisms, which are widespread and highly varied.
Bioinformatics is a discipline developed on the basis of biology, mathematics and computer science. It effectively acquires and analyzes biological data such as nucleic acid sequences and protein structures, so as to conduct comprehensive and accurate biological analysis. Bioinformatics is an extremely important tool to study the microorganisms, which are widespread and highly varied.   Bioinformatics in…

Nano-materials, which made of silicon, gallium arsenide and other semiconductor materials, possess many excellent properties.
Nano-magnetic materials In practice, most of the nanomaterials are artificial. Magnetic nano-materials made up of small-sized nano-particles and single-domain structures, so they have very special magnetic properties and high coercivity properties. Recording materials that made up of magnetic nanomaterials are not only possess good sound, image quality, and high SNR(signal to noise ratio), but also have several times recording density of?-Fe2O3. Superparamagnetic ferromagnetic nanoparticles can also be made into magnetic fluids for electroacoustic devices, damping devices, rotary seals, lubrication and beneficiation fields. Nano-ceramic materials Traditional ceramic…

Bachem, the Swiss pharmaceutical company, invested in a capacity expansion for large-scale production of oligonucleotide active pharmaceutical ingredients (APIs).
Each step within the company’s oligonucleotide manufacturing process uses unique, innovative equipment to deliver the highest-quality products and to increase sustainability. Tailor-made oligonucleotide synthesisers To ensure only the highest-quality oligonucleotide is supplied to customers, Bachem designed a large-scale oligonucleotide synthesiser. This synthesiser optimises the process of oligonucleotide synthesis and has been qualified for GMP batches from a 0.2 mol to 2 mol scale. One of the engineering solutions used for the innovative synthesiser is in-line mixing of dichloroacetic acid (DCA) and toluene. This solution is u…

SiSaf Ltd, an RNA delivery and therapeutics company, announces that it is initiating the U.S. FDA Regulatory process to obtain an Orphan Drug Designation for SIS-101-ADO, a siRNA therapeutic for patients with Autosomal Dominant Osteopetrosis Type 2 (ADO2), a rare genetic skeletal disorder. The request for Orphan Drug Designation and advancement through the complex regulatory process that SIS-101-ADO must undergo is being coordinated by CSSi LifeSciences, a leader in shepherding emerging therapeutics through federal approval processes.
The SIS-101-ADO combines an siRNA that suppress the expression of CLCN7, with SiSaf’s Bio-Courier® next generation silicon stabilized hybrid lipid nanoparticles (sshLNP) technology that addresses the limitations of other RNA delivery technologies by merging l…

Circular RNAs are closed-loop single-stranded RNA (ssRNA) that are structurally specific, and produced by the back-splicing process. CircRNAs can exist independently of proteins and are unaffected by exonucleases, and are widely found in plant and animal cells to regulate gene expression.
Circular RNAs are closed-loop single-stranded RNA (ssRNA) that are structurally specific, and produced by the back-splicing process. CircRNAs can exist independently of proteins and are unaffected by exonucleases, and are widely found in plant and animal cells to regulate gene expression.   Circular RNAs were first discovered by Sanger studying virus-like RNAs. And in 1991, Nigro stumbled upon an unusual novel RNA product. The low abundance of circRNA expression and the limitations of detection techno…

Methylation is one of the most classical epigenetic modifications in eukaryotes. DNA methylation regulates gene expression and has important implications in both growth and disease-related research. DNA methylation affects the maturation of germ cells or embryonic cells subject to specific gene expression. It has also been widely studied and applied to track aging and various forms of human disease. Various diseases, especially cancer have a pattern of DNA methylation that is not present in normal cells, resulting in differences in gene expression levels.
Methylation is one of the most classical epigenetic modifications in eukaryotes. DNA methylation regulates gene expression and has important implications in both growth and disease-related research. DNA methylation affects the maturation o…

Currently, the incidence of infectious diseases has increased globally, and the pathogens show a trend of diversification and complexity. Rapid and accurate pathogenic diagnosis, which enables early selection of sensitive anti-infective drugs or effective and targeted preventive and control measures, is the core of accurate prevention and treatment of infectious diseases. The epidemic of respiratory pathogens has been bringing a huge disease burden to human beings, as in the case of the global pandemic of novel coronavirus (SARS-CoV-2), which has seriously affected world health and economic development.
Currently, the incidence of infectious diseases has increased globally, and the pathogens show a trend of diversification and complexity. Rapid and accurate pathogenic diagnosis, which enabl…

As drug delivery vehicles, liposomes have low toxicity and immunogenicity, and the high biocompatibility and biodegradability of their components—phospholipids can increase the in vivo drug concentrations and protect drugs from degradation.
Due to the amphiphilic nature of lipids in liposomes, they can serve as candidate vehicles for drug delivery, and liposomes have received more attention than other systems because of their remarkable ability to deliver drugs to their target sites. Advantages of Liposomes as Drug Delivery Vehicles As drug delivery vehicles, liposomes have low toxicity and immunogenicity, and the high biocompatibility and biodegradability of their components—phospholipids can increase the in vivodrug concentrations and protect drugs from degradation. They can be easily mo…

As new drug development gravitates away from chemical synthesis towards new and costly biotech therapies and vaccines, the Quality function is becoming increasingly central to optimising manufacturing processes. Dr. Eduard Cayón of Rephine/ TDV outlines how Quality can add value for emerging and established biopharma companies.
The increasing change of focus in pharma, away from small chemical molecules towards biotech products and personalised therapies, is driving a change in the requirements of manufacturing sites, equipment and processes needed for this new environment. For young biotechs, scaling up production brings green-field challenges, while for more established pharma businesses a shift may be required to ensure that facilities are optimised for new ways of working. Across all of…

Liposomes vary in size, composition and charge and can be used as carriers for a wide range of drug molecules.
Liposomes are a novel drug delivery system (DDS). They are bimolecular vesicles that form spontaneously when phospholipids are dispersed in water. Liposomes vary in size, composition and charge and can be used as carriers for a wide range of drug molecules, such as chemotherapeutic agents, antimicrobial and antiviral drugs, antiparasitic drugs, genetic material, vaccines, therapeutic proteins, and anti-inflammatory agents. Liposomal formulations have unique advantages in both safety and efficacy, so they are one of the most rapidly developing formulations in recent years. In the past 30 years, research in the field of liposomes has made great progress. Various types…

A variety of newly developed peptide-based drugs are expected to become a promising new class of anti-tumor therapeutic drugs. This blog will give a brief introduction to anti-tumor drugs based on peptide form.
Peptide-based drug design has attracted much interest with the rapid growth of bioinformatics knowledge related to peptides and proteins. In the past, peptide-based drugs were not widely used due to some limitations such as low selectivity and rapid degradation of peptides in biological systems. Excitingly, novel peptide-based therapies emerged in recent years, showing excellent anti-tumor efficacy. A variety of newly developed peptide-based drugs are expected to become a promising new class of anti-tumor therapeutic drugs. This blog will give…

CPPs are believed to matter a lot in the treatment of animal and human diseases as more interdisciplinary research goes deeper and its potential effectiveness and specificity continue to be explored.
Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular intake and uptake of molecules ranging from nano-size particles to small chemical compounds to large fragments of DNA. As a versatile peptide, CPP has been widely used for diagnostic and therapeutic applications.   1. Oligo and siRNA Transport As an important means for targeted therapy of specific genes, oligonucleotides and siRNA can regulate protein activation and post-transcriptional gene expression. Besides, they’re also broadly utilized in the research of signal transmission systems, upstream and downstrea…

Epigenetics is an emerging field of research that focuses on the effects of environmental factors, such as infections, pollutants, stress, and long-term drug exposure, on an individual's genome. Epigenetic changes do not alter the structure of DNA, but do alter the way DNA is modified. Thereby, those changes include DNA methylation, histone modifications and non-coding RNAs. affects gene regulation.
Epigenetics is an emerging field of research that focuses on the effects of environmental factors, such as infections, pollutants, stress, and long-term drug exposure, on an individual's genome. Epigenetic changes do not alter the structure of DNA, but do alter the way DNA is modified. Thereby, those changes include DNA methylation, histone modifications and non-coding RNAs. affects gene regulat…

Since the first discovery of 16s rRNA sequences in environmental samples in 1990 and their value in microbial community research, we have entered the era of microbial community research in full swing. Today, community research has entered the high throughput sequencing era, and the mainstream of current research is at the intersection of next-generation amplicon sequencing and long-read amplicon sequencing.
Since the first discovery of 16s rRNA sequences in environmental samples in 1990 and their value in microbial community research, we have entered the era of microbial community research in full swing. Today, community research has entered the high throughput sequencing era, and the mainstream of current research is at the intersection of next-generation amplicon sequencing and long-read…

HLA, human leukocyte surface antigen, a series of tightly interlocking motifs on the short arm of human chromosome 6, is the central basis for the immune system to recognize and differentiate between allogeneic substances.
HLA, human leukocyte surface antigen, a series of tightly interlocking motifs on the short arm of human chromosome 6, is the central basis for the immune system to recognize and differentiate between allogeneic substances. It is highly polymorphic and corresponds to a complex acquired immune system. It is importantly associated with a variety of autoimmune diseases, tumors, and infectious diseases. Also, HLA plays a crucial role in transplants such as organ and bone marrow transplant and is associated with serious adverse reactions to many drugs. Therefore, HLA typing is…

The cell is the basic unit of life. Most current studies on the human genome, cancer or other fields are still conducted from the population level, and the results are often the mean value of gene expression in cell populations or only represent information on the vital activities of numerically dominant cells, while failing to accurately reflect much of the information on cellular heterogeneity and ignoring the variability of gene expression regulation between cells.
The cell is the basic unit of life. Most current studies on the human genome, cancer or other fields are still conducted from the population level, and the results are often the mean value of gene expression in cell populations or only represent information on the vital activities of numerically dominant cells, while failing t…

Recently, a paper called Cyclic Peptide Drugs Approved in Last Two Decades was published in RCS Chemical Biology. This work details the cyclic peptide drugs authorized for clinic use between 2001 and 2021 and reveals their future development trends.
Recently, a paper called Cyclic Peptide Drugs Approved in Last Two Decades was published in RCS Chemical Biology. This work details the cyclic peptide drugs authorized for clinic use between 2001 and 2021 and reveals their future development trends.   Romidepsin Romidepsin is a natural dicyclic peptide discovered in Bacillus purplicum (1994), consisting of a caprolactone cyclization and a pair of disulfide bonds, produced primarily by fermentation. Romidepsin(Isodax®) is a histone deacetylase inhibitor (HDACi) approved by the FDA in…

Cytokines are small soluble proteins that are secreted by immune cells and tissue cells to play a regulatory role between cells, including interferon (IFN), interleukin (IL), chemokines, colony-stimulating factors (CSF), tumor necrosis factors (TNF) and so on. Cytokines function in autocrine, paracrine or endocrine forms, and can regulate cell growth and differentiation, modulate immune response and participate in the development of various diseases such as inflammation by binding to the corresponding receptors.
Cytokines are small soluble proteins that are secreted by immune cells and tissue cells to play a regulatory role between cells, including interferon (IFN), interleukin (IL), chemokines, colony-stimulating factors (CSF), tumor necrosis factors (TNF) and so on. Cytokines function in…

Lipidomics, a platform developed by Creative Proteomics, is dedicated to providing cutting-edge mass spectrometry (MS)-based lipidomics services for biomedical research institutions, and biotechnological and pharmaceutical companies. Creative Proteomics Lipidomics launched the steroid analysis service capable of quantifying up to 38 steroids in biological matrices such as plasma, serum, tissue, cells, and cell culture media.
Lipidomics, a platform developed by Creative Proteomics, is dedicated to providing cutting-edge mass spectrometry (MS)-based lipidomics services for biomedical research institutions, and biotechnological and pharmaceutical companies. Creative Proteomics Lipidomics launched the steroid analysis service capable of quantifying up to 38 steroids in biological matrices such…